染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位

doi:10.11843/j.issn.0366-6964.2017.03.007

摘要/Abstract

摘要：

旨在研究染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位。采集黑色和白色各3只绵羊的背部皮肤组织，用qRT-PCR检测不同毛色皮肤中BPTF和BRG1 mRNA相对表达量，免疫组化技术定位分析，Western blotting半定量研究BPTF和BRG1蛋白相对表达量。qRT-PCR结果表明，BPTF基因在黑色绵羊皮肤中的mRNA相对表达量显著高于白色绵羊（P<0.05），BRG1基因在黑色绵羊皮肤中的mRNA相对表达量极显著高于白色绵羊（P<0.01）；Western blotting结果表明，黑色绵羊的皮肤中BPTF蛋白表达量显著高于白色绵羊（P<0.05），BRG1蛋白表达量极显著高于白色绵羊（P<0.01）；免疫组化结果表明，BPTF蛋白和BRG1蛋白在绵羊皮肤中毛囊的毛根鞘及毛球部均有表达。综上表明，BPTF和BRG1基因可能参与绵羊毛色形成过程。

Abstract:

The objective of this study was to explore the expression and localization of chromatin remodeling factor BPTF and BRG1 in black and white sheep skin tissues. Skins were collected surgically from the hindquarters of 3 black sheep and 3 white sheep. The expression level and distribution of BPTF and BRG1 were detected by quantitative real-time-polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of sheep. qRT-PCR and Western blotting results suggested that BPTF was expressed at significantly higher levels in black sheep compared with that of white sheep（P<0.05）; BRG1 was expressed at very significantly higher levels in black sheep compared with that in white sheep（P<0.01）. Immunohistochemistry demonstrated BPTF and BRG1 staining in the root sheath and dermal papilla in hair follicle of sheep skins. Therefore, it come to the conclusion that BPTF and BRG1 may involve in the coat color formation of sheep.

中图分类号:

S826.2

孙笑尉，张雪莲，李振，庞全海. 染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位[J]. 畜牧兽医学报, 2017, 48(3): 446-453.

SUN Xiao-wei, ZHANG Xue-lian, LI Zhen, PANG Quan-hai. The Expression and Localization of Chromatin Remodeling Factor BPTF and BRG1 in Black and White Sheep Skin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 446-453.

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https://www.xmsyxb.com/CN/Y2017/V48/I3/446

参考文献

［1］XIAO H, SANDALTZOPOULOS R, WANG H M, et al. Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions ［J］. Mol Cell, 2001, 8(3):531-543.
［2］KOLUDROVIC D,LAURETTE P,STRUB T,et al.Chromatin-remodelling complex NURF is essential for differentiation of adult melanocyte stem cells ［J］. PLoS Genet, 2015, 11(10): e1005555.
［3］DAR A A, NOSRATI M, BEZROOKOVE V, et al. The role of BPTF in melanoma progression and in response to BRAF-targeted therapy ［J］. J Natl Cancer Inst, 2015, 107(5): pii: djv034.
［4］XIONG Y, LI W, SHANG C, et al. Brg1 governs a positive feedback circuit in the hair follicle for tissue regeneration and repair ［J］.Dev Cell, 2013, 25(2):169-181.
［5］BAI J, MEI P, ZHANG C, et al. BRG1 is a prognostic marker and potential therapeutic target in human breast cancer ［J］. PLoS One, 2013, 8(3): e59772.
［6］BARKER N, HURLSTONE A, MUSISI H, et al. The chromatin remodelling factor Brg-1 interacts with beta-catenin to promote target gene activation ［J］. EMBO J, 2001, 20(17):4935-4943.
［7］LAURETTE P, STRUB T, KOLUDROVIC D, et al. Transcription factor MITF and remodeller BRG1 define chromatin organisation at regulatory elements in melanoma cells ［J］. Elife. 2015, 4: 10.7554/eLife.06857.001.
［8］KEENEN B, QI H, SALADI S V, et al. Heterogeneous SWI/SNF chromatin remodeling complexes promote expression of microphthalmia-associated transcription factor target genes in melanoma［J］. Oncogene, 2010, 29(1): 81-92.
［9］DAR A A, MAJID S, BEZROOKOVE V, et al. BPTF transduces MITF-driven prosurvival signals in melanoma cells ［J］. Proc Natl Acad Sci U S A, 2016, 113(22): 6254-6258.
［10］WEHRLE-HALLER B, WESTON J A. Soluble and cell-bound forms of steel factor activity play distinct roles in melanocyte precursor dispersal and survival on the lateral neural crest migration pathway［J］. Development, 1995, 121(3): 731-742.
［11］HENGGE U R, BAER A, BARDENHEUER W, et al. Towards a molecular profile of melanoma: Dysregulation of the Ras/Raf/ERK pathway［J］. J Clin Oncol, 2004, 22(14 Suppl): 7555.
［12］PENG U, WANG Z, PEI S, et al. ACY-1215 accelerates vemurafenib induced cell death of BRAF-mutant melanoma cells via induction of ER stress and inhibition of ERK activation［J］. Oncol Rep, 2016, 28: 10.3892.
［13］DAS A M, PESCATORI M, VERMEULEN C E, et al. Melanomas prevent endothelial cell death under restrictive culture conditions by signaling through AKT and p38 MAPK/ ERK-1/2 cascades［J］. Oncoimmunology, 2016 , 5(10): e1219826.
［14］SEONG Z K, LEE S Y, POUDEL A, et al. Constituents of cryptotaenia japonica inhibit melanogenesis via CREB- and MAPK-associated signaling pathways in murine B16 melanoma cells［J］. Molecules, 2016, 21(10): E1296.
［15］GODING C R. A picture of Mitf in melanoma immortality ［J］. Oncogene, 2011, 30(20):2304-2306.
［16］MOORE K J. Insight into the microphthalmia gene ［J］. Trends Genet, 1995, 11(11): 442-448.
［17］MCGILL G G, HORSTMANN M, WIDLUND H R, et al. Bcl2 regulation by the melanocyte master regulator Mitf modulates lineage survival and melanoma cell viability ［J］. Cell, 2002, 109(6): 707-718.
［18］HAQQ C, NOSRATI M, SUDILOVSKY D, et al. The gene expression signatures of melanoma progression ［J］. Proc Natl Acad Sci U S A, 2005, 102(17): 6092-6097.
［19］DE LA SERNA I L,OHKAWA Y,HIGASHI C,et al.The microphthalmia-associated transcription factor requires SWI/SNF enzymes to activate melanocyte-specific genes［J］.J Biol Chem,2006,281(29): 20233-20241.
［20］VACHTENHEIM J, ONDRUSOVA L, BOROVANSKY J. SWI/SNF chromatin remodeling complex is critical for the expression of microphthalmia-associated transcription factor in melanoma cells ［J］. Biochem Biophys Res Commun, 2010, 392(3): 454-459.
［21］SALADI S V, WONG P G, TRIVEDI A R, et al. BRG1 promotes survival of UV-irradiated melanoma cells by cooperating with MITF to activate the melanoma inhibitor of apoptosis gene ［J］. Pigment Cell Melanoma Res, 2013, 26(3): 377-391.
［22］KEENEN B, QI H, SALADI S V, et al. Heterogeneous SWI/SNF chromatin remodeling complexes promote expression of microphthalmia-associated transcription factor target genes in melanoma ［J］. Oncogene, 2010, 29(1): 81-92.
［23］LIN H, WONG R P, MARTINKA M, et al. BRG1 expression is increased in human cutaneous melanoma ［J］. Br J Dermatol, 2010, 163(3): 502-510.
［24］HORIKAWA T, NORRIS D A, JOHNSON T W, et al. DOPA-negative melanocytes in the outer root sheath of human hair follicles express premelanosomal antigens but not a melanosomal antigen or the melanosome-associated glycoproteins tyrosinase, TRP-1, and TRP-2 ［J］. J Invest Dermatol, 1996, 106(1): 28-35.
［25］BLAKW J A,ZIMAN M R. PAX3 transcripts in melanoblast development［J］.Dev Growth Differ, 2005,47(9): 627-635.
［26］NISHIMURA E K, GRANTER S R, FISHER D E.Mechanisms of hair garying: incomplete melanocyte stem cell maintenance in the niche ［J］. Science, 2005, 307(5710): 720-724.
［27］PETERS E M,TOBIN D J,BOTCHKAREVA N,et al.Migration of melanoblasts into the developing murine hair follicle is accompanied by transient e-Kit expression ［J］. J Histochem Cytochem, 2002, 50(6): 751-766.
［28］LEI T C,VIEIRA W D,HEARING V J.In vitro migration of melanoblasts maxtrix metalloproteinase-2:implications to vitligo therapy by photochemotherapy ［J］. Pigment Cell Res, 2002, 15(6): 426-432.
［29］BOTCHKAREVA N V, KHLGATIAN M, LONGLEY B J, et al. SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit ［J］. FASEB J, 2001, 15(3): 645-658.
［30］VAN NESTE D,TOBIN D J.Hair cycle and hair pigmentation: dynamic interactions and changes associated with aging ［J］. Micron, 2004, 35(3): 193-200.

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